Association of Nrf2 gene polymorphism (rs6721961) with semen parameters in primary male infertility cases in Pakistani population

Objective: To determine an association of single nucleotide polymorphism the NRF2 gene (RS6721961) with semen parameters primary male infertility patients in Pakistani population.
 Study Design: Comparative cross-sectional study.
 Place and Duration Study: Department Biochemistry, Islamic International Medical College, Rawalpindi, collaboration two private clinics (American Infertility Center, Rawalpindi Mother Child Hospital Research Institute, Mirpur, AJK) from Oct 2020 to Sep 2021.
 Methodology: A total 288 participants were included this study. There 144 diagnosed cases healthy fertile males, age ethnicity matched controls. Blood samples collected after obtaining written informed consent. DNA was extracted by Chelex TM Methodoogy. Multiplex PCR done respective allelic frequencies genotypes using specific primers.
 Results: no significant between volume semn colour infertile males (p-value 0.32 0.84, respectively). Out cases, 111 (77%) had normal sperm count, 29 (20.1%) oligospermia, while 4 (2.7%) azoospermia. observed concentration <0.001). 80 (55.5%) motility, 57 (39.5%) asthenospermia 7 (8.4%) necrospermia. strong motility <0.001).
 Conclusion: Single is significantly associated and......